Before the draft, it was determined that OT Kirk Barton, TB Michael DeMaria, FB Steve Fender, TB Maurice Hall, LB Joe Bradley, LB Mike D’Andrea, S Tyler Everett, CB Dustin Fox, DT Marcus Green, DL David Patterson and DL Joel Penton would all not play. TE Ryan Hamby, FB Branden Joe, SE Devin Jordan, DE Redgie Arden, WLB A.J. Hawk, WLB Curt Lukens, LB Trever Robinson and S Antonio Smith would all play limited reps due to injuries.
* WR -- (Scarlet assigned John Hollins, Gray assigned Bam Childress) Santonio Holmes (S), Roy Hall (G), Tony Gonzalez (S), Devin Jordan (G), Derek Harden (S), Brent Ullery (G), David Lisko (S), Wes Scott (G).
* C -- Nick Mangold (G), Steve Winner (S) and Justin Whaley (S).
* SLB -- Bobby Carpenter (S), Marcus Freeman (G) and Knake (G).
* QB -- Troy Smith (G), Justin Zwick (S), Todd Beckman (will play for both teams as needed).
* DE -- (Simon Fraser placed on Gray) Jay Richardson (S), Chris Schlichter (G, but later traded to Scarlet for Redgie Arden).
* TE -- (Jason Caldwell placed on Gray) Louis Irizarry (G), Ryan Hamby (S) and Jordan Hoewischer (S).
* OT -- (John McLaughlin placed on Gray) Rob Sims (S), Tim Schafer (G), T.J. Downing (S), Steve Rehring (G).
* Leo DE -- Mike Kudla (G), Marcel Frost (S), Reggie Smith (G), Redgie Arden (S, but later traded to Gray for Chris Schlichter).
* Safety -- Nate Salley (S), Donte Whitner (G), Brandon Mitchell (S), Rob Harley (G), Antonio Smith (S).
* DT -- Quinn Pitcock (G), Sian Cotton (S), Brandon Maupin (G), Derek Coker (S), Caesar Buie (G), Joel Penton (S).
* CB -- (Harlen Jacobs placed on Scarlet, Fox placed on Gray) Ashton Youboty (S), E.J. Underwood (G), Michael Roberts (S), De’Angelo Haslam (G).
* OG -- (Mike Kne placed on Scarlet) Doug Datish (G), Andree Tyree (S), R.J. Coleman (G), John Conroy (S), Danny Dye (G).
* TB -- (Lydell Ross and Michael DeMaria placed on Scarlet, Roshawn Parker and Maurice Hall placed on Gray) Antonio Pittman (S), Colby Staubs (G) and Matthew Johnson (G).
* MLB -- Anthony Schlegel (G), John Kerr (S).